COLL 427 |
| Anne L. Plant, Biomolecular Materials Group/Biotechnology Division/CSTL, Biomolecular Materials Group/Biotechnology Division/CSTL, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899-8313 |
| Self-assembled monolayers have been frequently used in the assembly of thin films of proteins. We have employed this approach for the assembly of thin films of monomeric and fibrillar Type 1 collagen, and have studied cell response on these surfaces. Smooth muscle cell phenotype is dependent on structural moieties from extracellular matrix proteins, like collagen, which initiate intracellular signaling pathways. Because intracellular signaling pathways are extremely complex, the use of self-assembly reduces variability and allows assessment of the inherent distribution in cell signaling responses to surface chemistries. The mean area of smooth muscle cells grown on monomeric collagen is greater than that of cells on fibrillar collagen by a factor of ~2.2. The distribution of sizes in these two populations is extremely reproducible but different from each other. How such a distribution might arise will be discussed with respect to other parameters. co-authors include John T. Elliott, Kurt J. Langenbach, and Alessandro Tona. Biotechnology Division, National Institute of Standards and Technology, Gaithersburg MD and Dept. of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence RI. |
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Adamson Award Symposium Honoring Dave Allara and Ralph Nuzzo
Division of Colloid and Surface Chemistry |