COLL 35 |
| Harvey W. Blanch and Sean M. Ferree. Department of Chemical Engineering, University of California, Tan Hall, Berkeley, CA 94720-1462 |
| Electrophoresis has been a powerful and ubiquitous tool for the separation and analysis of DNA molecules. However our understanding of the mechanisms of separation, beyond the traditional sieving and reptation models for gel electrophoresis, is incomplete, particularly in dilute polymer solutions. We examine the electrophoresis of restriction fragment DNA molecules in dilute and ultra-dilute polymer solutions. DNA undergoes large conformational changes during electrophoresis, and these conformational dynamics must be included in models of the electrophoretic separation. We present epifluorescent microscopy data of the electrophoretic stretching of tethered DNA molecules to probe the influence DNA dynamics on the separation process and discuss a theory to describe these single molecule DNA stretching dynamics |
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ACS Award in Colloid and Surface Chemistry Symposium Honoring Clay Radke
Division of Colloid and Surface Chemistry |