COLL 415 |
| Luis G Cascão-Pereira1, Harvey W. Blanch2, and Clayton J. Radke2. (1) Genencor International, 925 Page Mill Road, Palo Alto, CA 94304, (2) Department of Chemical Engineering, University of California at Berkeley, Gilman Hall 201, Berkeley, CA 94720-1462 |
| Protein adsorption at fluid/fluid interfaces is important in biopharmaceutical manufacture, stabilization and delivery, in biocatalysis, and food technology. Upon adsorption, proteins may undergo conformational changes, which stabilize the interfacial protein layers against rupture. The behavior of b-casein and bovine serum albumin (BSA) upon adsorption at the air/water interface will be discussed. The surface forces arising upon confinement of adsorbed proteins layers between air/water interfaces have been measured. Data suggest protein conformational changes at the interface lead to the formation of elastic networks. The nature of these interfacial protein networks has been characterized by interfacial rheology measurements. The mechanisms responsible for protein interfacial layer stability are thus different to those present in layers formed by low-molecular weight molecules, such as detergents.
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ACS Award in Colloid and Surface Chemistry Symposium Honoring Clay Radke
Division of Colloid and Surface Chemistry |