Hydration interactions between apoferritin molecules and the phase behavior of the solution

COLL 368

Peter G. Vekilov, Dimiter N. Petsev, Simon Brandon, and Panagyotis Katsonis. Department of Chemical Engineering, University of Houston, Houston, TX 77204-4004
We link the specificity of phase behavior – lack of liquid-liquid separation and temperature-independent solubility – to intermolecular interactions in the solution characterized using light scattering. Results indicate in the presence of Na+ and Cd2+ ions the intermolecular interaction potential has a repulsive part due to Na+ assisted hydration sphere build-up at separations between 0.5 and 3 nm, and a short range (<0.2 - 0.1 nm) attractive part due to Cd2+ mediated bonds. To link the found structured potential to the phase diagram we carried out Monte Carlo simulations, using Gibbs-Duhem and Gibbs Ensemble techniques, with an intermolecular potential having a minimum and a local maximum at separations longer than those of the minimum. Increasing the height of the maximum resulted in steeper liquidus lines, and eventually, in temperature-independent solubility. Another consequence of the increasing maxima was the shift of the liquid-liquid separation line to lower temperatures, not accessible in an experiment.
 

Colloidal and Molecular Electro-Optics

Division of Colloid and Surface Chemistry
The 225th ACS National Meeting, New Orleans, LA, March 23-27, 2003