COLL 179 |
| Surangkhana Martwiset, Mamle Quarmyne, and Wei Chen. Department of Chemistry, Mount Holyoke College, 50 College Street, South Hadley, MA 01075 |
| After almost two decades of research, it is now well known that poly(ethylene glycol) (PEG)-containing surfaces are resistant to protein adsorption, and are thus biocompatible. In this research, other neutral hydrophilic molecules are evaluated as PEG alternatives in terms of protein resistance. Dextran has been adsorbed to a silicon-supported covalently attached monolayer prepared from tridecafluoro-1,1,2,2-tetrahydrooctyldimethylchlorosilane. The adsorbed amount is controlled by adsorption time, polysaccharide concentration and solution ionic strength. Crosslinking with glutaraldehyde renders the adsorbed dextran stable to aqueous solutions. D-glycero-D-gluo-heptose, D-maltose, and dextran have been grafted to amine-containing silicon-supported covalently attached monolayers. Protein resistance of these surfaces containing hydrophilic molecules varying in identity, quantity (density and chain length) and conformation is assessed by adsorption of albumin, lysozyme, and collagen. All of the surfaces are characterized by contact angle analysis, ellipsometry, x-ray phtoelectron spectroscopy and atomic force microscopy. |
|
Adsorption of Macromolecules at Liquid-Solid Interfaces
Division of Colloid and Surface Chemistry |