Micropatterning biomembrane functions using light

COLL 425

Atul N. Parikh, Chanel K Yee, and Meri L. Amweg. Department of Applied Science, University of California, Davis, Department of Applied Science, Davis, CA 95616
We describe a simple, generic method for micro-patterning supported bilayer membranes(sBLMs) and selected membrane functions simply using a physical mask and deep UV light. In particular, Spatially-directed exposure of sBLMs to UV light in the 184-257 nm range, in conjunction with ensuing production of atomic oxygen and ozone, provides stable microsocopic sBLM patterns by removal of exposed lipids (and proteins) leaving behind disjointed membrane patches (or "holes") at optically defined locations and pre-defined geometries on the substrate surface. Refunctionalization of the lipid-free regions provides new opportunities to engineer membrane composition as well as create bifunctional arrays of lipid or protein functions.
 

Adamson Award Symposium Honoring Dave Allara and Ralph Nuzzo

Division of Colloid and Surface Chemistry
The 225th ACS National Meeting, New Orleans, LA, March 23-27, 2003