COLL 158 |
| Rik ter Veen1, Mats Jönsson2, Jesper Gantelius1, Ulf Lindberg2, and Karin D. Caldwell3. (1) Center for Surface Biotechnology, University of Uppsala, BMC, Box 577, 751 23 Uppsala, Sweden, (2) Department of Materials Science, University of Uppsala, Ångström Laboratory, Box 534, 751 21 Uppsala, Sweden, (3) Center for Surface Biotechnology, Uppsala University, BMC, Box 577, 75123 Uppsala, Sweden |
| Electrical Field-Flow Fractionation (ElFFF) is an analytical technique that separates particles by an electrical field perpendicular to a laminar flow field [1]. With increasing field strength, smaller particles can be analyzed. In ElFFF, the field strength depends on the channel thickness [2] and on the effectivity of the electrode system. We present a micronized ElFFF channel with optimized electrodes and detection and injection in the channel. In current electrode configurations, the effective field strength is approximately 1% of the nominal field strength due to a slow or an ion-producing electrode reaction. The new optimized electrode system reacts fast with protons in the carrier liquid. This leads to a strong increase in effective field strength. This should make it possible to analyze particles as small as proteins. 1. K.D. Caldwell, Y.-S. Gao, Anal. Chem., 48, 1834 (1993) 2. B. Gale, et al., Anal. Chem., 73, 2345 (2001) |
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Colloidal and Molecular Electro-Optics
Division of Colloid and Surface Chemistry |