COLL 14 |
| Albena Ivanisevic, Heriberto Hernandez, Meng Yu, and Nyamjav Dorjderem. Biomedical Engineering and Chemistry, Purdue University, 1296 Potter Bldg, West Lafayette, IN 47907 |
| The identification, fabrication and evaluation of biomimetic templates hold great potential for variety of applications. The generation of templates on these substrates is achieved using an appropriately modified atomic force microscope (AFM) tip. Colloids or cells immobilized onto a surface, and can serve as “functional pixels” as a result of being modified to have either a negatively- or positively-charged outer layer. AFM tips are modified in a variety of ways to have positively- or negatively-charged molecules at their ends. This allows one to remove selectively either certain layers of the “functional pixels” on the surface or even entire “functional pixels” by removing the AFM tip according to a predetermined blueprint. Such removal is highly dependent upon specific adhesion forces and can be monitored using topography and force curves data during the process. By changing the nature of biological and chemical species used for surface and tip modification, we can enhance or decrease these forces when necessary. The success of this patterning was evaluated using AFM, High-resolution Transmission Electron Microscopy (HR-TEM) and Scanning Electron Microscopy (SEM), depending on the substrate and surface modification chosen. |
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Nanoscale Imaging of Biological Systems
Division of Colloid and Surface Chemistry |