The Gutenberg Bibles hold an exalted place in publishing history as the earliest examples of books that were printed rather than hand copied. The printing of the Gutenberg Bibles was completed in 1455. Of approximately 180 copies, substantial portions of 48 survive in collections around the world.

	DISCERNING PALETTES Chaplin (left) and Clark led the team that used Raman microscopy to study the Gutenberg Bibles.
	COURTESY OF ROBIN CLARK

Clark and his coworkers have rectified that situation. They used Raman spectroscopy to examine the illuminations (decorations) and inks in a copy of the Gutenberg Bible held at the British Library, known as the King George III copy, and compared its pigment palette with those of six other copies held elsewhere in England, France, and Germany (Anal. Chem. 2005, 77, 3611). Previous studies of the bibles have focused on the composition of the printing inks and paper.

After the bibles were printed, many parts, including the headlines, rubrics, and major capitals, needed to be added by hand. In addition, some of the copies were richly illuminated with elaborate borders and other illustrations. Because these additions varied, they are believed to have been performed locally to the buyer's specifications rather than coordinated by Johann Gutenberg's workshop.

The pigments used for the hand-drawn portions of the bibles are a potential source of information about the books and their time, including possible trade routes and artists' preferences. A standard model book offered guidance in terms of style and pigments, but the diversity in the finished products suggests that the guidelines weren't always followed.

"We wanted to determine how diverse the palette of different Gutenberg Bibles might be, given that they were not all illuminated in the same city or at the same date," says lead author Tracey D. Chaplin, a postdoc in Clark's lab.

The analysis is part of a larger project at the British Library to study illuminated "incunabula" (15th-century printed materials) and the technical and artistic developments that occurred as printing began to supersede handwritten manuscripts. "At the time the bibles were printed, the intention was that these early printed books should physically resemble the traditional book form, the handwritten manuscript," says David Jacobs, a senior conservation officer at the British Library in London and one of Clark's collaborators.

Raman spectroscopy is particularly well-suited for nondestructive analysis of the bibles, compared with other methods such as X-ray fluorescence or diffraction, because it provides molecular information rather than just elemental analysis. "The large size of the bible itself can put limits on instrument geometry, as the book cannot be opened completely flat," Chaplin says. The use of fiber-optic cables for the spectroscopic analysis makes the awkward size and shape of the book less of a problem because samples need not be taken from the books. In any case, the team received permission to remove small samples from the gutters and margins of copies held at other places for analysis back at the British Library.

The spectroscopic results showed that the palettes of all seven copies are similar and "rather limited," Chaplin says. The palette for the King George III copy contains nine pigments (plus gold leaf), three of which were found in all copies examined and two others that were found in six of the copies. The other copies also contain pigments that weren't found in the King George III copy.

The copy housed at the Niedersächsische Staats und Universitätsbibliothek, in Göttingen, Germany, has the most extensive palette of the seven copies examined, with 16 pigment-related materials identified. That copy was also printed on vellum--a more expensive material--rather than paper. Vellum is not necessarily an indication of luxury, however, as it was sometimes chosen for its durability.

For modern purposes, knowing which pigments were used is critical for conservation efforts, Clark says. "Use of incorrect pigments, even if they are of the correct color, could destroy any work of art through adverse chemical reactions. It is vitally important that these critical facts are brought to the attention of the art world as well as to those in science," he says. "Pigment degradations--common with lead and copper pigments--are simply chemical reactions, which need to be recognized for what they are and studied as such."

COURTESY OF THE BRITISH LIBRARY

GOOD BOOK The palette of the King George III copy of the Gutenberg Bible, housed at the British Library, contains nine pigments. The first page of the prologue, containing St. Jerome's introductory letter, is shown here.